Identification of PPARdelta transcriptional complex by photo-crosslinking

2014 Fiscal Year Final Research Report

• Project/Area Number: 25670035
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Pharmacology in pharmacy
• Research Institution: The University of Tokyo
• Principal Investigator: TANAKA Toshiya 東京大学, 先端科学技術研究センター, 特任准教授 (20396930)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Keywords: 核内受容体 / プロテオミクス / クロスリンク / PPARδ

Outline of Final Research Achievements

We have carried out to establish a transcription factor complex identification method combining cross-linking and high-resolution mass spectrometry using PPARδ as a model transcription factor.
We found photo-reactive amino acids induce cell toxicity. Formaldehyde cross-linking causes decrease in coverage of target protein and increase in non-specific protein binding. Knockdown of SATB1, which is identified as macrophage-specific PPARδ interact protein, by shRNA reduces macrophage-specific PPARδ target gene induction by PPARδ agonist.
These results suggest the possibility of the identification of native PPARδ transcriptional complex using combination of cross-linking and high-resolution mass spectrometry.

Free Research Field

核内受容体医学