Establishment of hepatocyte model from induced pluripotent stem (iPS) cells for exploring uremic toxin production inhibitors

2015 Fiscal Year Final Research Report

• Project/Area Number: 25670080
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Medical pharmacy
• Research Institution: Kumamoto University
• Principal Investigator: Saito Hideyuki 熊本大学, 医学部附属病院, 教授 (40225727)
• Co-Investigator(Kenkyū-buntansha): JONO Hirofumi 熊本大学, 医学部附属病院, 准教授 (40515483) ; SHIRAKI Nobuaki 東京工業大学, 生命理工学研究科, 准教授 (70448520)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 尿毒症物質 / 肝細胞 / iPS細胞 / 硫酸転移酵素 / 腎障害

Outline of Final Research Achievements

This study aimed to establish a screening system for exploring inhibitors of uremic toxin production using human hepatocyte model cells derived from induced pluripotent stem (iPS) cells, as a hepatic production system for sulfate-conjugated uremic toxins. By using four different culture medium for differentiation induction, AFP-positive cells, a marker cell of prodromal hepatocyte, were detected 11 days after the culture initiation, thereby indicating that it was possible to induce the differentiation of endodermal frozen stock cells into genealogical hepatocytes. Newly established human hepatocytes from iPS cells could be a useful tool for screening inhibitors for indoxyl sulfate production in the human liver.

Free Research Field

薬物毒性学