Continuous pH measurement of interstitial fluid and clarification of pathogenic mechanism of insulin resistance by ISFET pH sensor

2014 Fiscal Year Final Research Report

• Project/Area Number: 25670111
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: General physiology
• Research Institution: Kyoto Prefectural University of Medicine
• Principal Investigator: MARUNAKA Yoshinori 京都府立医科大学, 医学(系)研究科(研究院), 教授 (00127036)
• Co-Investigator(Kenkyū-buntansha): NIISATO Naomi 京都府立医科大学, 医学研究科, 准教授 (00237645) ; MIYAZAKI Hiroaki 京都府立医科大学, 医学研究科, 講師 (30360027) ; HOSOGI Shigekuni 京都府立医科大学, 医学研究科, 助教 (30405261)
• Co-Investigator(Renkei-kenkyūsha): HIDESHIMA Sho 早稲田大学, 大学院理工学術院, 助教 (10580433)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Keywords: pH / 糖尿病 / 組織間質液

Outline of Final Research Achievements

A) Developments of collection methods of interstitial fluid and methods approaching to interstitial fluid. We succeeded in two-day insertion of syringe with outer diameter of 0.80 mm and inner diameter of 0.57 mm in interstitial fluid space.
B) pH measurements of interstitial fluid. Even if we tried to obtain the interstitial fluid using the syringe, we could not get the interstitial fluid due to clogged inner spaces of the syringe.Therefore, we tried to directly measure pH of interstitial fluids using micro pH meters,ISFET pH sensor and antimony pH sensor.We could not obtain any stable pH value of interstitial fluid using ISFET pH sensor, however stable values of interstitial fluid pH were obtained by antimony pH sensor.Using antimony pH sensor, we obtained pH of 7.21 ± 0.03 in diabetic rats and pH of 7.36 ± 0.02 in non-diabetic rats（n = 6; p < 0.05）.

Free Research Field

イオン環境と生体機能