Suggested common mechanism underlying between human Muse cells and planarian neoblasts

2014 Fiscal Year Final Research Report

• Project/Area Number: 25670135
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: General medical chemistry
• Research Institution: Tohoku University
• Principal Investigator: DEZAWA Mari 東北大学, 医学(系)研究科(研究院), 教授 (50272323)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Keywords: 多能性幹細胞 / 線維芽細胞 / 間葉系幹細胞 / 結合組織 / 進化

Outline of Final Research Achievements

We identified HsDj2 in human Muse cells in immunoprecipitation using specific antibody to planarian neoblast. Splice variants expressed in human Muse cells were surveyed and 9 kinds of spriced forms were identified including full length. By comparing expression pattern in Muse and non-Muse MSC, 2 out of 9 subtypes were suggested to be selectively expressed only in Muse cells. Furthermore, by introducing siRNAs, exon 4 suppression in Muse cells promoted neural differentiation, and exon 6 suppression hepatocyte-lineage differentiation, suggesting that HsDj2 is pertinent to Muse cell pluripotency as well as to differentiation.

Free Research Field

医歯薬学