2015 Fiscal Year Final Research Report
Analysis of dynamic functions of membrane microdomains by linkage of glycosphingolipids on the cell membrane and transcriptional regulation
| Project/Area Number |
25670141
|
|---|
| Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
General medical chemistry
|
|---|
| Research Institution | Chubu University (2015)
Nagoya University (2013-2014) |
|---|
Principal Investigator |
FURUKWA Koichi 中部大学, 生命健康科学部, 教授 (80211530)
|
|---|
| Project Period (FY) |
2013-04-01 – 2016-03-31
|
| Keywords | ガングリオシド / 転写因子 / セクレターゼ / ミクロドメイン / 脂質ラフト |
|---|
| Outline of Final Research Achievements |
Regulatory mechanisms by which interaction between GD3 and its associating molecule, neogenin on the cell surface of melanoma cells were analyzed with focus on the intracytoplasmic domain of neogenin (Ne-ICD). When a protease inhibitor was added to the culture medium, increased levels of Ne-ICD was detected in GD3+ cells, while it was hard to detect when a gamma-secretase inhibitor was added, suggesting that Ne-ICD was generated by recruiting gamma-secretase to lipid rafts by GD3 expression.
By ChIP-sequencing, target genes that are driver by Ne-ICD have been identified, and those genes have been up-regulated by the introduction of Ne-ICD cDNA.
|
| Free Research Field |
生化学、糖鎖生物学
|
