2015 Fiscal Year Final Research Report
Development of the tumor marker detection method by RNA aptamer
Project/Area Number |
25670151
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
General medical chemistry
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Research Institution | Kinki University (2015) Nagoya City University (2013-2014) |
Principal Investigator |
|
Research Collaborator |
UENO Yoshihito 岐阜大学, 応用生物科学部, 教授 (20250467)
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Project Period (FY) |
2013-04-01 – 2016-03-31
|
Keywords | RNAアプタマー |
Outline of Final Research Achievements |
Sphingosine 1-phosphate (S1P) is combined with a sphingosine 1-phosphate receptor (S1PR) emerging in the cell surface and shows bioactivity. Five subtypes of S1PR have been identified so far, and these are tissue-specific. Recently, it has been revealed that S1PR affected the malignant transformation of cancer deeply, and the positioning as the tumor marker became important. Therefore I used high polymer material called " RNA" with the artificial ligand which could be specifically combined with each subtype of S1PR and tried that it produced wounds. However, I succeeded in the construction of the S1PR surface presentation cell and tried whole Cell SELEX, but dominant RNA aptamer was not provided.
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Free Research Field |
RNA生化学
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