Control of vascular barrier integrity by MTM family of phosphatidylinositol 3-phosphate phosphatase

2014 Fiscal Year Final Research Report

• Project/Area Number: 25670162
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Pathological medical chemistry
• Research Institution: Kanazawa University
• Principal Investigator: TAKUWA Yoh 金沢大学, 医学系, 教授 (60171592)
• Co-Investigator(Renkei-kenkyūsha): YOSHIOKA Kazuaki 金沢大学, 医学系, 助教 (80333368)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Keywords: ホスホイノシチド / ホスファターゼ / PI3-キナーゼ / 小胞 / 血管 / 内皮

Outline of Final Research Achievements

We studied phosphatidylinositol 3-phosphate (PI-3-P) phosphatase in vascular endothelial cells. Among 14 isoforms of MTM family members, we identified one isoform which was relatively abundantly expressed in endothelial cells. Fluorescent imaging showed the expression of GFP-tagged phosphatase in endosomes. The phosphatase negatively regulated cellular PI-3-P level and was involved in the regulation of endosomal movement. The PI-3-P phosphatase controlled migration, cell-cell adhesion and morphogenesis of endothelial cells through regulating PI-3-P level.

Free Research Field

血管医学、病態医化学、生理学、細胞生物学、脂質生化学