Detection of estrogen receptor dimerization patter in breast carcinoma tissues.

2014 Fiscal Year Final Research Report

• Project/Area Number: 25670174
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Human pathology
• Research Institution: Tohoku University
• Principal Investigator: SASANO HIRONOBU 東北大学, 医学(系)研究科(研究院), 教授 (50187142)
• Co-Investigator(Renkei-kenkyūsha): MIKI Yasuhiro 東北大学, 災害科学国際研究所, 講師 (50451521)
• Research Collaborator: IWABUCHI Erina 東北大学, 大学院医学系研究科, 技術補佐員
• Project Period (FY): 2013-04-01 – 2015-03-31
• Keywords: 乳癌 / 近接ライゲーションアッセイ / エストロゲン受容体 / ダイマー

Outline of Final Research Achievements

Examination of estrogen receptors (ER) is important to determine appropriate treatment options for breast cancer. ERα and ERβ dimers bind DNA with comparable affinities as either homo- or heterodimers to the same estrogen response elements. ER dimers have been identified by molecular technique but no in situ methodologies have been reported. Proximity Ligation Assay (PLA) could demonstrate the molecular proximity of two proteins in situ in tissue specimens. In this study, we established detection method of ER dimerization using PLA between specific antibodies for ERα and ERβ in breast cancer cell lines and breast carcinoma tissues.

Free Research Field

医歯薬学