2015 Fiscal Year Final Research Report
Establishment of genetically engineered mouse creation foundation based on a new concept
| Project/Area Number |
25670176
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Human pathology
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| Research Institution | Kyushu University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
HRADA Yui 九州大学, 大学院薬学研究院, 助教 (00608507)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Keywords | 遺伝子操作マウス / センダイウイルスベクター / RNAヘリカーゼ |
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| Outline of Final Research Achievements |
Embryonic stem cells (ES cells) are derived from the inner cell mass of blastocysts (early embryos). These cells are pluripotent, which means that they can develop into almost any type of tissue. ES cells are used for more precise modifications of the mouse genome. We have previously developed Sendai virus (SeV) vectors that replicate in the form of negative-sense single-stranded RNA in the cytoplasm of infected cells and do not go through a DNA phase. Here we report that the SeV-mediated gene transfer into murine ES cells was very efficient and stable. This technique makes it possible to insert as well as remove or modify DNA sequences. Knock-out, knock-in and conditional mutant mice can be produced with this method.
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| Free Research Field |
遺伝子治療学・血管病態学
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