2014 Fiscal Year Final Research Report
In vivo observation of stemness acquisition in differentiated cancer cells
| Project/Area Number |
25670177
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Human pathology
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| Research Institution | Nara Medical University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
KITADAI Yasuhiko 広島大学, 医歯薬学大学院, 准教授 (10304437)
CHIHARA Yoshitomo 奈良県立医科大学, 医学部, 講師 (40405395)
FUJIMOTO Yoshio 奈良県立医科大学, 医学部, 教授 (90192731)
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| Co-Investigator(Renkei-kenkyūsha) |
TAKAKI Miyako 奈良県立医科大学, 医学部, 特任教授 (00033358)
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Keywords | がん幹細胞 / 幹細胞分化 |
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| Outline of Final Research Achievements |
Dedifferentiation or acquisition of stemness was examined in mouse cancer cells. Expression of CD133 and nucleostemin in CT26 cells showed that differentiations of dormant cancer stem (DCS) to proliferating cancer stem (PCS) and PCS to differentiated cancer cells (DCC) were found in 3.5% and 5.6%, respectively. In contrast, dedifferentiations of DCC to PCS and PCS to DCS were found in 1.7% and 1%, respectively. In in vivo observation of KT-5 cells in mouse brain showed stem-related ALP activity was disappeared in 16%, whereas appeared in 1%. These findings suggest that the dedifferentiation or acquisition of stemness might be a common phenomenon in cancer. The biological meaning and clinical importance should be examined in further studies.
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| Free Research Field |
人体病理
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