IL-1beta production depending on size of insoluble material generated in vivo

2014 Fiscal Year Final Research Report

• Project/Area Number: 25670192
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Experimental pathology
• Research Institution: Kyoto University
• Principal Investigator: INABA Kayo 京都大学, 生命科学研究科, 教授 (00115792)
• Co-Investigator(Kenkyū-buntansha): TAKAHARA Kazuhiko 京都大学, 大学院生命科学研究科, 講師 (90301233)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Keywords: 微細粒子 / IL-1β / アミロイド β / 炎症

Outline of Final Research Achievements

Small particles/aggregates are known to provoke inflammatory response of macrophages (Mφ). In this study, latex beads (LxB) of 1000 nm and 20 nm, but not 100 nm in diameter, were shown to induce IL-1β production of Mφ-like microglia in the brain. Although aggregates of amyloid β (Aβ) are believed as causative agent of Alzheimer disease, only nano-sized Aβ oligomers, but not large Aβ fibril, stimulated IL-1β production of MG. In addition, the induction of IL-1β by nano-sized Aβ oligomers was dependent on reactive oxygen species (ROS) and protease cathepsin B. These results suggest that size/form of small particles/aggregates affects IL-1β production of MG.

Free Research Field

免疫生物学