Development of new targeting-type adjuvant molecule derived from bacteria

2014 Fiscal Year Final Research Report

• Project/Area Number: 25670209
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Bacteriology (including mycology)
• Research Institution: The University of Tokyo
• Principal Investigator: MIMURO Hitomi 東京大学, 医科学研究所, 准教授 (80396887)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Keywords: 細菌 / ヘリコバクターピロリ

Outline of Final Research Achievements

The purpose of the project is to identify molecules of Helicobacter pylori that are responsible for an adjuvanticity on gut-associated lymphoid tissues (GALT), and to consider the possibility as new GALT-targeting adjuvant molecules. We have performed shotgun LC-MS/MS proteomics analysis of proteins from Peyer’s patches-transmissive and non-transmissive bacterial outer membranes, and identified the candidate molecules. We have successfully selected the candidate of high importance by bioinformatics method, and produced deletion mutant strains of the factors. The in vitro and in vivo analysis of the deletion mutants revealed that the factors have potential to regulate ability of targeting Peyer’s patches.

Free Research Field

細菌学