The identification of host molecules in PV transmission into brood brain barrier

2014 Fiscal Year Final Research Report

• Project/Area Number: 25670221
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Virology
• Research Institution: Microbial Chemistry Research Foundation
• Principal Investigator: MIZUTANI Taketoshi 公益財団法人微生物化学研究会, 微生物化学研究所, 研究員 (00376617)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Keywords: 血液脳関門 / ポリオウイルス

Outline of Final Research Achievements

Polio　virus (PV) infects into human cells by binding to CD155 and entries into the nerve system through unknown mechanisms. In this study, I examined a responsible element in Polio virus (PV) for the transmission into nerve system through the blood brain barrier (BBB) using mouse blood endothelial cells (MBEC4).
I determined that the transferrin receptor (TfR) as a candidate for a host molecule for PV transmission into BBB, by showing PV capsid protein, VP1 interacts with TfR in vitro. I identified each responsible domain of the interaction between VP1 and TfR using in vitro binding assay. Further examination shows that PV capside protein, VP1 is an important molecule for the interaction wiht TfR and PV transmission into MBEC4. Together, these results help to understand PV transmission into the nerve system.

Free Research Field

ウイルス学