The pathogenesis of flaviviral encephalitis bases on the immunological

2014 Fiscal Year Final Research Report

• Project/Area Number: 25670223
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Virology
• Research Institution: National Institute of Infectious Diseases
• Principal Investigator: KURANE ICHIRO 国立感染症研究所, その他部局等, 所長 (90278656)
• Co-Investigator(Kenkyū-buntansha): SUZUKI Ryuji 独立行政法人国立病院機構, 相模原病院臨床研究センター, 室長 (70373470) ; TAKASAKI Tomohiko 国立感染症研究所, ウイルス第一部, 室長 (20221351)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Keywords: ウイルス / 基礎医学 / 微生物 / 脳神経 / 免疫学

Outline of Final Research Achievements

We identified brain-infiltrating T cells associated with a fatal outcome of Japanese encephalitis (JEV) infection in mice. Five each of dying mice and surviving mice were used. Virus titers were at the similar levels between two groups. Cytokine patterns in the brain revield a higher ratio of Th1-related cytokine genes in dying mice. The expression levels of CD3, CD8, CD25, and CD69 increased in JEV-infected mice relative to mock-infected mice. However, expression levels of these cell-surface markers did not differ between the two groups. T cells expressing VA8-1, VA10-1, and VB2-1 increased in both groups. However, the dominant T-cell clones as defined by CDR3 amino acid sequence differed between the two groups. The results indicate that the outcome of JEV infection, death or survival, was determined by qualitative differences in infiltrating T-cell clones with unique CDR3 amino acid sequences.

Free Research Field

ウイルス学