Novel viral vector by using activation of innate immune responses

2014 Fiscal Year Final Research Report

• Project/Area Number: 25670225
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Virology
• Research Institution: 独立行政法人医薬基盤研究所
• Principal Investigator: YASUTOMI YASUHIRO 独立行政法人医薬基盤研究所, 霊長類医科学研究センター, センター長 (90281724)
• Co-Investigator(Kenkyū-buntansha): WATANABE Kenta 山口大学, 共同獣医学部, 助教 (20582208)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Keywords: 免疫 / ワクチン / 感染症 / ウイルスベクター / 結核

Outline of Final Research Achievements

We assessed the effectiveness of a novel mucosal vaccine using recombinant human parainfluenza type 2 virus (rHPIV2) as a vaccine vector. rHPIV2 showed the expression of inserted Ag in respiratory epithelial cells. M gene eliminated rHPIV2, which is replication-incompetent, incorporated cDNA of Ag85B gene from Mycobacterium bovis (rHPIV2-Ag85B) elicited Ag85B-specific immune responses. Moreover, rHPIV2-Ag85B showed protective effects against M.tuberculosis infection. We also constructed HN protein, which is receptor molecule to infection with respiratory epithelial cell, deleted rHPIV2. These findings provide evidence for the possibility of rHPIV2 as a novel mucosal vaccine vector.

Free Research Field

免疫学