Development of a PET imaging system for cancer diagnosis using Zr-89 lableled anti-mesothelin scFv

2014 Fiscal Year Final Research Report

• Project/Area Number: 25670273
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Laboratory medicine
• Research Institution: Okayama University
• Principal Investigator: MATSUURA Eiji 岡山大学, 医歯(薬)学総合研究科, 教授 (20181688)
• Co-Investigator(Kenkyū-buntansha): TAKENAKA Fumiaki 岡山大学, 大学院医歯薬学総合研究科, 助教 (10642522) ; SASAKI Takanori 岡山大学, 大学院医歯薬学総合研究科, 助教 (10461253)
• Research Collaborator: KOBAYASHI Kazuko 岡山大学, 大学院医歯薬学総合研究科, 助教 (20304298) ; KITA Shoichi 株式会社医学生物学研究所 (70524205)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Keywords: Zr-89 / がん / PET / 低分子抗体 / 画像診断

Outline of Final Research Achievements

For the purpose of developing an antibody-based PET imaging agent of cancer, we produced scFv against mesothelin (MSLN). MSLN is a 40-kDa cell differentiation-associated glycoprotein appeared with carcinogenesis and a potential target for a wide range of cancer diagnosis and therapy. Anti-MSLN scFv was conjugated with a chelator deferoxamine (DFO) and radiolabeled with zirconium-89. 89Zr-labeled anti-MSLN scFv was administered intravenously to NCI-H226 tumor bearing mice and imaged with PET/CT up to 48 h post administration followed by terminal biodistribution studies. 89Zr-labeled anti-MSLN scFv was highly accumulated in tumor and a PET image with the highest contrast of tumor to background was obtained at 48 h post injection. These results indicate the high potential of 89Zr-labeled anti-MSLN scFv as a PET probe for the detection of cancer.

Free Research Field

免疫学、病態検査学、分子イメージング