Elucidation of factors causing sepsis and establishment of prediction method by clinical analysis and molecular bacteriological approach

2016 Fiscal Year Final Research Report

• Project/Area Number: 25670277
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Laboratory medicine
• Research Institution: Gihu University of Medical Science
• Principal Investigator: NAKAYAMA Akifumi 岐阜医療科学大学, 保健科学部, 教授 (70536721)
• Co-Investigator(Renkei-kenkyūsha): MATSUYOSHI hiroko 関西学院大学, 理工学部, 助教 (10448772)
• Project Period (FY): 2013-04-01 – 2017-03-31
• Keywords: 敗血症 / 大腸菌 / 病原因子 / 髄膜炎

Outline of Final Research Achievements

In the analysis using Escherichia coli strains derived from bacteremia, urinary tract infection, healthy subjects classified by clinical analysis, 9 pathogenic genes （fimH, afa, iha, cvaC, kpsMT, ompT, aer, usp, ibeA）were predominantly detected in strains derived from bacteremia. In the search of novel pathogenic genes, the analysis of whole genome sequence of E. coli strain derived from neonatal meningitis, homology search against BLAST (https://blast.ncbi.nlm.nih.gov/Blast.cgi) and Pfam (http://pfam. sanger.ac.uk/) database were performed. As a result, 12 genes were found in which protein functions are unknown and similar structural motifs are not recognized in known proteins. Furthermore, these genes showed almost no possibility in 88 E. coli strains derived from bacteremia, urinary tract infection, and healthy individuals. From these results, we suggested that 12 unknown function genes have an important role in the passage of the blood brain barrier of E. coli.

Free Research Field

臨床微生物学