Analyses of intrinsic and extrinsic miRNA regulating proliferation of human iPS cell-derived hepatoblats.

2014 Fiscal Year Final Research Report

• Project/Area Number: 25670373
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Gastroenterology
• Research Institution: Tokai University
• Principal Investigator: KAMIYA Akihide 東海大学, 創造科学技術研究機構, 准教授 (30321904)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Keywords: 幹細胞 / iPS細胞 / 肝臓 / miRNA

Outline of Final Research Achievements

Human iPS cells can be differentiated into hepatocytic cells by the serial cytokine stimulation. We previously found that hepatic progenitor-like cells (HPCs) were enriched in the CD13+CD133+ cell fraction of iPS-differentiated cells. In this study, we focused on the cell surface molecules and analyzed the characteristics of human iPS cell-derived HPCs. CD221 (IGF1 receptor) was down-regulated during the long-term culture. After the replating step, positive and negative cells of these surface markers were cultured. Then, CD221+ cells had high proliferative ability compared with CD221- cells. The proliferative ability of HPCs was suppressed by the neutralizing antibody and specific inhibitor of CD221. In addition, IGF-1 and IGF-2 were produced by mouse embryonic fibroblast, which are used as feeder cells in our culture system. Now, we analyze expression of miRNA regulating hepatoblast proliferation and differentiation.

Free Research Field

細胞生物学