Role of phopholipid metabolism in the pathophysiology of renal injury

2014 Fiscal Year Final Research Report

• Project/Area Number: 25670407
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Kidney internal medicine
• Research Institution: Tokyo Medical and Dental University
• Principal Investigator: SUGANAMI Takayoshi 東京医科歯科大学, 医歯(薬)学総合研究科, 寄附講座教授 (50343752)
• Co-Investigator(Renkei-kenkyūsha): SETOU Mitsutoshi 浜松医科大学, 医学部, 教授 (20302664) ; AOE Seiichiro 大妻女子大学, 家政学部, 教授 (90365049) ; MORI Kiyoshi 京都大学, 大学院医学研究科, 特定准教授 (60343232)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Keywords: 急性腎不全 / 質量顕微鏡 / リン脂質 / 尿細管

Outline of Final Research Achievements

Imaging mass microscope is a novel technique that is a combination of an optical microscope with a mass spectrometer, which enables us to know what kind of molecules are located in the region of interest. Kidney, which is complex anatomically and physiologically, may be a good target of imaging mass spectrometry. In this study, we induced renal ischemia-reperfusion injury as an acute kidney injury model and observed area-specific changes in quality and quantity of phosphatidylcholine. Consistently, transcriptome analysis revealed that mRNA expression of a series of enzymes of phosphatidylcholine metabolism was harmoniously regulated under these conditions. These observations suggest that imaging mass spectrometry is a powerful technology to investigate the role of local metabolic changes in response to pathophysiologic stresses.

Free Research Field

腎臓内科学