コムギ胚芽無細胞蛋白質合成によるIgG4関連腎疾患の抗原同定と腎病理形態の解析

2014 Fiscal Year Annual Research Report

• Project/Area Number: 25670411
• Research Institution: Ehime University
• Principal Investigator: 増本 純也 愛媛大学, プロテオサイエンスセンター, 教授 (20334914)
• Co-Investigator(Kenkyū-buntansha): 上原 剛 信州大学, 医学部, 准教授 (80402121) ; 下条 久志 信州大学, 医学部, 講師 (40324248)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Keywords: IgG4 / 膜性腎症 / 無細胞蛋白質合成 / H. Pylori / P. Gingibalis / EBウィルス

Outline of Annual Research Achievements

H.pylori, P. gingivalisのゲノムライブラリーとLaji細胞株とサイトメガロウィルス感染組織からmRNAを抽出し、逆転写反応によって得られたcDNAからpDONR221-cDNAライブラリーを構築した。このライブラリーを基に、ビオチン結合無細胞蛋白質合成発現ライブラリーを得て、コントロール血清と高IgG4血清の無作為スクリーニングを行っている。スクリーニングはamplified luminescent proximity homogeneous assayで行っているが、現在までのところ再現性のある陽性シグナルを得られていない。原因となる病原体が異なることも考えられるため、引きつづき、様々な病原体感染組織のライブラリーを構築し、スクリーングを行っていきたい。

Research Products

• [Journal Article] Epstein-Barr virus-related MTX-LPD in rheumatoid arthritis patients exhibits a viral pattern of the CD64 and CD35 expression on neutrophils: three case reports. (2015)
  • Author(s): Mokuda S, Miyazaki T, Saeki Y, Masumoto J, Kanno M, Takasugi K
  • Journal Title: Mod Rheumatol Volume: 25 Pages: 166-168
  • DOI: 10.3109/14397595.2013.875641
  • Peer Reviewed
• [Journal Article] Dysregulated mature IL-1β production in familial Mediterranean fever. (2015)
  • Author(s): Migita K, Izumi Y, Fujikawa K, Agematsu K, Masumoto J, Jiuchi Y, Kozuru H, Nonaka F, Shimizu T, Nakamura T, Iwanaga N, Furukawa H, Yasunami M, Kawakami A, Eguchi K.
  • Journal Title: Rheumatology (Oxford) Volume: 54 Pages: 660-665
  • DOI: 10.1093/rheumatology/keu359
  • Peer Reviewed
• [Journal Article] Development of tumor-specific caffeine-potentiated chemotherapy using a novel drug delivery system with Span 80 nano-vesicles. (2015)
  • Author(s): Nakata H, Miyazaki T, Iwasaki T, Nakamura A, Kidani T, Sakayama K, Masumoto J, Miura H
  • Journal Title: Oncol Rep Volume: 33 Pages: 1593-1598
  • DOI: 10.3892/or.2015.3761
  • Peer Reviewed
• [Journal Article] IL-18 serum concentration is markedly elevated in typical familial Mediterranean fever with M694I mutation and can distinguish it from atypical type (2015)
  • Author(s): Yamazaki T, Shigemura T, Kobayashi N, Honda K, Yazaki M, Masumoto J, Migita K, Agematsu K
  • Journal Title: Mod Rheumatol Volume: 印刷中 Pages: 印刷中
  • Peer Reviewed
• [Journal Article] IL-1 as a target in inflammation (2015)
  • Author(s): Ito Y, Kaneko N, Iwasaki T, Morikawa S, Kaneko K, Masumoto J.
  • Journal Title: Endocr Metab Immune Disord Drug Targets Volume: 印刷中 Pages: 印刷中
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Corresponding Author of manuscript "The proto-oncogene survivin splice variant 2B is induced by PDGF and leads to cell proliferation in rheumatoid arthritis fibroblast-like synoviocyte (2015)
  • Author(s): Mokuda S, Miyazaki T, Ito Y, Yamasaki S, Inoue H, Guo Y, Kong W, Kanno M, Takasugi K, Sugiyama E, Masumoto J
  • Journal Title: Scientific Reports Volume: 印刷中 Pages: 印刷中
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Defect of suppression of inflammasome-independent interleukin-8 secretion from SW982 synovial sarcoma cells by familial Mediterranean fever-derived pyrin mutations (2014)
  • Author(s): Sugiyama R, Agematsu K, Migita K, Nakayama J, Mokuda S, Ogura F, Haraikawa K, Okumura C, Suehiro S, Morikawa S, Ito Y, Masumoto J.
  • Journal Title: Mol Biol Rep Volume: 41 Pages: 545-553
  • DOI: 10.1007/s11033-013-2890-y.
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Tocilizumab improved clinical symptoms of a patient with systemic tophaceous gout who had symmetric polyarthritis and fever: An alternative treatment by blockade of interleukin-6 signaling (2014)
  • Author(s): Mokuda S, Kanno M, Takasugi K, Okumura C, Ito Y, Masumoto J
  • Journal Title: SAGE Open Medical Case Reports Volume: 2 Pages: 250313X13519774
  • DOI: 10.1177/2050313X13519774
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Familial Mediterranean fever: genotype-phenotype correlations in Japanese patients (2014)
  • Author(s): Migita K, Agematsu K, Yazaki M, Nonaka F, Nakamura A, Toma T, Kishida D, Uehara R, Nakamura Y, Jiuchi Y, Masumoto J, Furukawa H, Ida H, Terai C, Nakashima Y, Kawakami A, Nakamura T, Eguchi K, Yasunami M, Yachie A.
  • Journal Title: Medicine (Baltimore) Volume: 93 Pages: 158-164
  • DOI: 10.1097/MD.0000000000000029
  • Peer Reviewed
• [Journal Article] Serum amyloid A induces NLRP-3-mediated IL-1β secretion in neutrophils. (2014)
  • Author(s): Migita K, Izumi Y, Jiuchi Y, Kozuru H, Kawahara C, Nakamura M, Nakamura T, Agematsu K, Masumoto J, Yasunami M, Kawakami A, Eguchi K.
  • Journal Title: PLoS One Volume: 9 Pages: e96703
  • DOI: 10.1371/journal.pone.0096703
  • Peer Reviewed
• [Journal Article] Microvasculature of carotid atheromatous plaques: hemorrhagic plaques have dense microvessels with fenestrations to the arterial lumen. (2014)
  • Author(s): Kurata M, Nose M, Shimazu Y, Aoba T, Kohada Y, Yorioka S, Suehiro S, Fukuoka E, Matsumoto S, Watanabe H, Kumon Y, Okura T, Higaki J, Masumoto J
  • Journal Title: J Stroke Cerebrovasc Dis Volume: 23 Pages: 1440-1446
  • DOI: 10.1016/j.jstrokecerebrovasdis.2013.12.003
  • Peer Reviewed
• [Presentation] Defect of suppression of inflammasome-independent interleukin-8 secretion from sw982 synovial sarcoma cells by familial mediterranean fever-derived pyrin mutations (2014)
  • Author(s): Masumoto J
  • Organizer: 16th Biennial Meeting of the European Society for Immunodeficiencies
  • Place of Presentation: Plagh, Czech Republic.
  • Year and Date: 2014-10-31 – 2014-11-02