Identification of antigens in IgG4-related kidney diseases by wheat germ-free protein synthesis system

2014 Fiscal Year Final Research Report

• Project/Area Number: 25670411
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Kidney internal medicine
• Research Institution: Ehime University
• Principal Investigator: MASUMOTO Junya 愛媛大学, プロテオサイエンスセンター, 教授 (20334914)
• Co-Investigator(Kenkyū-buntansha): UEHARA Takeshi 信州大学, 医学部, 准教授 (80402121) ; SHIMOJO Hisashi 信州大学, 医学部, 講師 (40324248)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Keywords: 膜性腎症 / 腎生検 / 病理

Outline of Final Research Achievements

Biotinylated protein syntheses from Helicobactor pylori, Porphyromonas gingivalis, and human B-cell lymphoma cell line Raji cells were performed by wheat germ cell-free protein synthesis system. Using these proteins as probes, immunofluorescence staining was performed using biopsy specimens in cases of membranous glomerulonephropathy, interstitial nephritis, and normal control. The fluorescence signal was overlapped with IgG fluorescence signal in some cases. Now the study about that these observations can be seen in other cases is going on.

Free Research Field

病理学