Evaluation of aging by using cell-free nucleic acid

2014 Fiscal Year Final Research Report

• Project/Area Number: 25670451
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Hematology
• Research Institution: Osaka University
• Principal Investigator: KANAKURA YUZURU 大阪大学, 医学(系)研究科(研究院), 教授 (20177489)
• Co-Investigator(Kenkyū-buntansha): ORITANI Kenji 大阪大学, 大学院医学系研究科, 准教授 (70324762) ; ICHII Michiko 大阪大学, 大学院医学系研究科, 寄附講座助教 (30633010) ; SAITOH Norimitsu 大阪大学, 大学院医学系研究科, 特任助教(常勤) (30597399)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Keywords: 老化 / 造血幹細胞 / 分化 / Wnt / クロマチン

Outline of Final Research Achievements

We established transgenic chimera mice to produce soluble Frizzled-related protein (sFRP)-5 under the immunoglobulin-kappa promoter. Overexpression of sFRP-5 inhibited B lymphocyte differentiation after the level of common lymphoid progenitors, leading to massive decrease of B lymphocytes in spleen. On the other hand, sFRP-5 deficient mice showed significant decrease of hematopoietic stem cells (HSC) when the mice were stimulated by estrogen. We established reporter mice, which monitor Special AT-rich sequence binding protein 1 (SATB1) gene expression. HSC contained two populations divided by SATB1-positivity, and the SATB1-positive cells had less HSC characters and more lymphocyte production than SATB1-negative cells.
Therefore, we successfully clarified in vivo roles of sFRP-5 and SATB1 in the lymphocyte production. Our results provide useful information to understand HSC aging.

Free Research Field

血液内科