The exploration of cognate antigens, which induce drug or metal allergy by using soluble TCR

2015 Fiscal Year Final Research Report

• Project/Area Number: 25670503
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Dermatology
• Research Institution: Osaka University
• Principal Investigator: Arase Noriko 大阪大学, 医学(系)研究科(研究院), 特任研究員 (30360481)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 可溶型TCR / 金属アレルギー / 薬剤アレルギー / HLA

Outline of Final Research Achievements

Recently, association between severe drug eruption and specific HLA allele has been identified, suggesting major role of T cell-immunity. T cell is also involved in metal allergy besides the activation of innate immunity. Whereas molecular mechanism how these small molecules to induce T cell response remains unclear. We considered that we could determine the nature of antigens by using recombinant solubilized T cell receptor αβ. At first, we developed recombinant TCR αβ chains specific for the certain peptide antigen. These TCR αβ chains react specifically to the peptide on antigen presenting cells. Next we established metal specific T cell clones, and confirmed the specificity of them. Thereafter, we established solubilized TCR αβ chains and analyzed the specificity. Unfortunately, these soluble TCR did not react specifically to the metal antigens. From these observations, it was revealed that method to produce soluble TCR for metal antigen need to be further optimized.

Free Research Field

皮膚科学　免疫学