2015 Fiscal Year Final Research Report
Development of Vaccine for Hansen's Disease-Targeting the M.leprae Molecular Mechanism for Entry into Nasal Mucosal Epithelial Cells
| Project/Area Number |
25670505
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Dermatology
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| Research Institution | Kitasato University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
SATO Naoya 北里大学, 医学部, 助教 (50276119)
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| Research Collaborator |
DAHLAN Haslindah
Viesta fadlitha beby
IDRIS Irfan
Fatulrachman
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Keywords | らい菌 / ワクチン / 抗mec抗体 / mce蛋白 / らい菌侵入抑制 / 侵入蛋白 |
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| Outline of Final Research Achievements |
We aim at investigating the invasion suppressing effect of various antibodies targeted against different regions of the M. leprae’s peptide for the nasal mucosal membrane invasion. 316-532bp (106-177A.A.) are considered to be the invasion-active region. This region was further divided into four sub-regions: 106-129A.A., 130-151A.A., 152-162A.A., and 163-177A.A.. A hyperimmune antisera was prepared for each of these sub-regions. Recombinant E. coli expressing the entire region including 130-151 amino acids in the M. leprae mce1A locus, that is,106-177 amino acids, were used to observe any changes in the M. leprae’s ability to enter the nasal membrane cells, where changes were caused by the treatment with antibodies. The observation revealed that the hyperimmune antisera prepared using 106-129A.A. and 130-151A.A. as immunogen had a strong invasion suppression effect. The hyperimmune antisera prepared with 152-162A.A. as immunogen did not show any invasion suppression effect.
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| Free Research Field |
皮膚免疫学 細菌学
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