Challenge to revival of steatotic liver graft by machine perfusion preservation

2015 Fiscal Year Final Research Report

• Project/Area Number: 25670555
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: General surgery
• Research Institution: Kyoto University
• Principal Investigator: Hata Koichiro 京都大学, 医学(系)研究科(研究院), 助教 (90523118)
• Co-Investigator(Kenkyū-buntansha): UEMOTO Shinji 京都大学, 大学院医学研究科, 教授 (40252449)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 脂肪肝 / 灌流保存 / 肝移植 / 脱脂肪化

Outline of Final Research Achievements

Almost 50% macrosteatotic liver was induced by dietary protocol in rat. At first, we confirmed that subnormothermic (20-25℃) machine perfusion preservation (SMP) protects steatotic livers from preservation/ reperfusion injury compared with static cold preservation, in terms of oxidative stress, mitochondrial damage, and microcirculatory disturbance, reslted in maintenance of functional & morphological viability.
In the next step, we investigated defatting power of SMP with drug facilitating fatty acid β oxidation and triglyceride secretion, like carnitine and peroxisomal proliferator-activated receptor. However, SMP didn't show little effect on defatting. Increase perfusion temperature and usage of oxygen carrier may be required fo defatting.

Free Research Field

肝移植