New organ preservation method under pressurized, non-frozen condition: Preclinical study using CLAWN miniature swine

2014 Fiscal Year Final Research Report

• Project/Area Number: 25670557
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: General surgery
• Research Institution: Kagoshima University
• Principal Investigator: YAMADA Kazuhiko 鹿児島大学, 医用ミニブタ・先端医療開発研究センター, 教授 (40241103)
• Co-Investigator(Kenkyū-buntansha): SAHARA Hisashi 鹿児島大学, 医用ミニブタ・先端医療開発研究センター, 准教授 (90452333)
• Research Collaborator: KOBAYASHI Atsushi
• Project Period (FY): 2013-04-01 – 2015-03-31
• Keywords: 移植・再生医療 / 前臨床実験 / 高圧 / 臓器保存 / 腎移植 / 心移植 / ミニブタ

Outline of Final Research Achievements

To extend organ preservation time, we attempted to establish a new preservation method in a stable unfrozen state below its freezing point by pressurizing the solution. To clarify the effects of high pressure on the kidney and heart, we performed kidney/heart transplantation in CLAWN miniature swine using the grafts preserved under high pressure (kidney: 60-120 MPa; heart: 30-60 MPa). Both kidney and heart grafts preserved under pressurized, subzero non-frozen condition had sufficient function after orthotopic kidney/heterotopic heart transplantation indicating that this method could be the novel strategy to extend organ preservation time.

Free Research Field

移植・再生医療