In vivo and vitro noble differentiation of induced pluripotent stem cell derived cardiomyocytes

2014 Fiscal Year Final Research Report

• Project/Area Number: 25670594
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Cardiovascular surgery
• Research Institution: Osaka University
• Principal Investigator: MIYAGAWA Shigeru 大阪大学, 医学(系)研究科(研究院), 特任准教授(常勤) (70544237)
• Co-Investigator(Kenkyū-buntansha): TODA Koichi 大阪大学, 大学院医学系研究科, 准教授 (40379235) ; YOSHIOKA Daisuke 大阪大学, 医学部附属病院, 助教 (40645959) ; SAITO Tetsuya 大阪大学, 大学院医学系研究科, 特任助教 (10644891)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Keywords: iPS細胞 / 心不全 / 心筋細胞

Outline of Final Research Achievements

The established differentiation of cardiomyocytes derived from iPS cells are expected to result in better efficacy of cell transplantation therapy. The differentiating cardiomyocytes were transplanted onto adult nude rate hearts. In the transplanted iPS cardiomyocytes in vivo, cardiac specific marker was much more expressed than the cardiomyocytes cultured in vitro. This result suggests that the environment that imitates the condition of cardiomyocytes differentiation promote the differentiation of iPS-CMs. The mechanical stretch modulated cell alignment and maturation gene expression in vitro.
The differentiation of cardiomyocytes derived IPS cells might be promoted in vitro by mechanical stretching.

Free Research Field

心臓血管外科学