Effect of transplanting normal human dermal fibroblasts (NHDF) in the rat facial nerve palsy model

2014 Fiscal Year Final Research Report

• Project/Area Number: 25670711
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Otorhinolaryngology
• Research Institution: Yamagata University
• Principal Investigator: KAKEHATA Seiji 山形大学, 医学部, 教授 (90261619)
• Co-Investigator(Kenkyū-buntansha): WATANABE Tomoo 山形大学, 医学部, 講師 (60344793) ; ITO Tsukasa 山形大学, 医学部, 助教 (50344809) ; FURUKAWA Takatoshi 山形大学, 医学部, 非常勤講師 (80466630)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Keywords: 顔面神経麻痺 / ヒト皮膚線維芽細胞 / 機能再生 / ラット

Outline of Final Research Achievements

Objective: To determine the effect of transplanting normal human dermal fibroblasts (NHDF) in the rat facial nerve palsy model. Methods: Eight weeks old Wister male rats (250g) were used. Facial nerves were transected at buccal branch and marginal branch in 6 mm length under general anesthesia. Polimer tubes filled with NHDF plus matrigel were grafted between the cut nerves. Wisking amplitude was measured to determine the facial nerve function. Result: Wisking amplitude was gradually recovered in 8 week. However, the effect of NHDF was not significant.

Free Research Field

耳鼻咽喉科学