2015 Fiscal Year Final Research Report
Micro-anatomy atlas using quantitative protein expression analysis
Project/Area Number |
25670750
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
Plastic surgery
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Research Institution | Kobe University |
Principal Investigator |
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Co-Investigator(Kenkyū-buntansha) |
NOMURA TADASHI 神戸大学, 医学部附属病院, 特命講師 (30529566)
SAKAKIBARA SHUNSUKE 神戸大学, 医学研究科, 特定助教 (50444592)
TAKASU HIROYUKI 神戸大学, 医学研究科, 特定助教 (40566022)
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Project Period (FY) |
2013-04-01 – 2016-03-31
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Keywords | 顔面神経損傷 / Hn-1 |
Outline of Final Research Achievements |
Whole rat brain mRNA was extracted and amplified a part of the rat (Wistar rat) Hn-1 gene by the polymerase chain reaction. After performing the sequence analysis, we confirmed that this was known Hn-1 gene with homology. cRNA probe was constructed and in situ hybridization was performed for a rat brain slice after the unilateral facial nerve damage using this cRNA probe. Expression of Hn-1 mRNA was observed at facial nerve nucleus and trigeminal nerve nucleous on the damaged side.
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Free Research Field |
形成外科学
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