Induction of dermal papilla cells from human iPS cells

2014 Fiscal Year Final Research Report

• Project/Area Number: 25670756
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Plastic surgery
• Research Institution: Research Institute, International Medical Center of Japan
• Principal Investigator: OKOCHI Hitoshi 独立行政法人国立国際医療研究センター, その他部局等, 部長 (30185235)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Keywords: 毛乳頭細胞

Outline of Final Research Achievements

Human dermal papilla cells (hDPC) were reported to express Sox2, Klf4, c-Myc which are key components for generating or maintaining iPS cells. Based on the similarity between hDPC and iPS cells, I hypothesized that suppressing the expression of the Oct3/4 gene in iPS cells may facilitate the differentiation of DPC from iPS cells. After I designed three different shRNA against Oct3/4 gene and applied them to the hiPS cells (253G:a gift from CiRA), I confirmed the expression level of the Oct3/4 gene was less than 30% of control group. Although I detected the expression of several genes specific for ectoderm, mesoderm and endoderm, I could not detect the up-regulation of DPC specific gene expression, such as versican. On the other hand I found the synergistic effect of FGF2 and PDGFAA for the growth of DPC, because addition of FGF2 to the culture media of DPC up-regulated the expression of PDGFα receptor. These results were published in Journal Dermatological Science.

Free Research Field

再生医学