Cell therapy using multilineage differentiating stress enduring (MUSE) cell for acute lung injury

2015 Fiscal Year Final Research Report

• Project/Area Number: 25670759
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Emergency medicine
• Research Institution: Tokyo Medical and Dental University
• Principal Investigator: UCHIDA Tokujiro 東京医科歯科大学, 医歯(薬)学総合研究科, 准教授 (40262183)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 急性肺傷害

Outline of Final Research Achievements

Multilineage differentiating stress enduring cells were isolated from a human dermal fibroblast cell line. The isolated cells were soated by SSEA3 positivity, and they maintained positivity of CD105 as a surface marker. When these cells were cultivated in Methocult H4100, they formed colony shaped cell spheres. However, we could not show the therapeutic effect of these cells in mice acute lung injury model, probably because of technical failure in the maintenance of the phenotype of MUSE cells. Further studies are needed to establish efficacy of cell therapy using MUSE cells.

Free Research Field

麻酔科学