Development of a new therapeutic method by genome-editing

2014 Fiscal Year Final Research Report

• Project/Area Number: 25670775
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Morphological basic dentistry
• Research Institution: Kyoto University (2014); Tokyo Medical and Dental University (2013)
• Principal Investigator: NAKAGAWA Ichiro 京都大学, 医学(系)研究科(研究院), 教授 (70294113)
• Co-Investigator(Kenkyū-buntansha): MARUYAMA Fumito 京都大学, 大学院医学研究科微生物感染症学分野, 准教授 (30423122)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Keywords: Ａ群レンサ球菌 / CRIPSR / TALEN / ZFN / 増殖抑制

Outline of Final Research Achievements

In this study, we tried to apply the Genome Editing method on the basis of the spacer sequence information of CRISPR. The Cas9 / CRISPR system of the group A streptococci (GAS) with various spacer sequences of the chromosomal DNA were constructed, and chromosomal fragments with homologous sequence were also introduced in another compatible plasmid. CRISPR/Cas system could clearly inhibit the growth of E. coli strain with matched pair of spacer sequence and the chromosomal fragment. However, the TALEN system and ZFN system could not inhibit the growth of E. coli completely. We also construct the CIRPSR/Cas9 expression plasmid for GAS. The growth of bacteria is completely inhibited by introducing the plasmid with spacer sequence of the homologous sequence of the its chromosome. These observations indicated that the CIRPSR/Cas9 system can be used not only for the destruction of foreign DNA but also its chromosome.

Free Research Field

細菌学