Establishment of the culture system to be able to maintain the development of growth / reproduction nervous system.

2015 Fiscal Year Final Research Report

• Project/Area Number: 25670782
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Morphological basic dentistry
• Research Institution: Ohu University (2014-2015); Showa University (2013)
• Principal Investigator: Hajime Imai 奥羽大学, 歯学部, 准教授 (90291343)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 長期全胚培養法 / GnRHニューロン / 摂食中枢 / 視索前野 / 鼻上皮 / 移動 / 前方中軸中内胚葉 / SHH

Outline of Final Research Achievements

GnRH-neurons originates from nasal epithelium and forms a network of feeding center. The aim of this study is to elucidate the inductive potencies of anterior axial mesendoderm (AME) for the determination of migratory pathway for GnRH neuron. We performed the loss of function with both surgical ablation of AME and a neutralizing antibody of Shh（5E1）in E9.5 rat embryos. The embryos were cultured for 72-96 hours in the whole embryo culture（WEC）. The surgical ablation resulted in the lack of transcripts of down-stream genes, such as Fgf-8 and Bmp-2, in nasal epithelium (NP). Moreover, the application of 5E1 reduced the detection levels of transcription factors relating with neurogenesis, e.g. Nkx2.1. Furthermore, we demonstrated the appearance of the immunoreactive cells against anti-GnRH antibody only in NP-derived vomeronasal organ. Shh contributes to the migration of GnRH-neuron through Gli3. These results suggest that Shh signaling is related to migration of GnRH-neuron.

Free Research Field

発生生物学