Cellular conversion from non- skeletal to skeletal cells by using transactivation MyoD domain

2014 Fiscal Year Final Research Report

• Project/Area Number: 25670784
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Morphological basic dentistry
• Research Institution: Tsurumi University
• Principal Investigator: NIFUJI Akira 鶴見大学, 歯学部, 教授 (00240747)
• Co-Investigator(Kenkyū-buntansha): ARAKI Ryoko 国立研究開発法人放射線医学総合研究所, 研究基盤センター, 室長 (40392211) ; NAKASHIMA Kazuhisa 鶴見大学, 歯学部, 講師 (90252692) ; IDENO Hisashi 鶴見大学, 歯学部, 学部助手 (40435699)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Keywords: 遺伝子 / 分化 / 転写因子

Outline of Final Research Achievements

Sets of specific transcription factors may induce cellular conversion from one committed cell to another. In the mesenchylmal cell lineages, the transcription factor MyoD can induce non-muscle cells to muscle cells. It is also reported that co-transfection of Sox9 with other sets of transcription factors may induce chondrocytes from non- chondrocytes. However reproducible and highly efficient cellular conversion from non- skeletal to skeletal cells is still challenging. In this study, we examined whether cellular conversion from non- skeletal to skeletal cells can be improved by using transactivation domain (TAD) of MyoD. Addition of TAD to Runx2 and sox9 enhanced activity of transcriptional transactivation. Cellular conversion rates by those factors did not change significantly, suggesting that additional factors or improvement of experimental condition are required for the cellular conversion of non- skeletal cells.

Free Research Field

発生学　薬理学