Drug-delivery-system through ion channels

2014 Fiscal Year Final Research Report

• Project/Area Number: 25670785
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Functional basic dentistry
• Research Institution: Tohoku University
• Principal Investigator: WAKAMORI Minoru 東北大学, 歯学研究科(研究院), 教授 (50222401)
• Co-Investigator(Renkei-kenkyūsha): YOSHIDA Takashi 東北大学, 歯学研究科, 助教 (30455795)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Keywords: イオンチャネル

Outline of Final Research Achievements

TRPV1 channel is activated by capsaicin and is permeable to Na+, K+ and Ca2+. Concentration-response relationship for capsaicin was obtained from the HEK293 cells expressing recombinant TRPV1 channel. The threshold concentration and EC50 of capsaicin were 0.03 and 0.22 uM, respectively. FM1-43, a positively charged organic ion, was a permeant when the TRPV1 channel was activated by 1 uM capsaicin, indicating that TRPV1 channel pore size is wide enough to pass the 452 Da FM1-43. When the extracellular Ca2+ was removed, the FM1-43 could not enter the cells through the TRPV1 channel. These results suggest that the TRPV1 channel pore is dilated by capsaicin but extracellular Ca2+ regulates the dilation.

Free Research Field

歯科薬理学