2014 Fiscal Year Final Research Report
Exploration of seeds for development of a new drug targeting Bone-Vascular-Spleen axis
| Project/Area Number |
25670793
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Functional basic dentistry
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| Research Institution | Matsumoto Dental University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
NINOMIYA Tadashi 松本歯科大学, 総合歯科医学研究所, 講師 (00360222)
NAKAMICHI Yuko 松本歯科大学, 総合歯科医学研究所, 講師 (20350829)
NAKAMURA Midori 松本歯科大学, 歯学部, 准教授 (90278177)
UEHARA Syunsuke 松本歯科大学, 歯学部, 講師 (90434480)
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Keywords | 破骨細胞 / 骨芽細胞 / マクロファージ / 脾臓 / RANKL / M-CSF / IL-34 |
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| Outline of Final Research Achievements |
(1)To clarify the role of spleen in bone metabolism, M-CSF-deficient mice and wild-type mice were subjected to splenectomy or a sham-operation. Splenectomy completely suppressed osteoclastogenesis in M-CSF-deficient mice. In contrast, splenectomy moderately suppressed osteoclastogenesis in wild-type mice.
(2)Comparing the gene expression profiles of IL-34-positive and IL-34-negative vascular endothelial cells identifies differentially expressed genes. We focused on top-ranked genes which possess leading signal peptide for secretion and membrane-anchoring.We are now addressing functional analyses of these genes.
(3)To elucidate mechanisms underlying the age-associated increase of IL-34 expressions in several tissues, we tried to establish the culture system for IL-34-positive vascular endothelial cells. However, the expression level of IL-34 gradually decreased in long-term culture.
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| Free Research Field |
口腔生化学
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