Identification and dissection of ATR substrates

2016 Fiscal Year Final Research Report

• Project/Area Number: 25701005
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Partial Multi-year Fund
• Research Field: Risk sciences of radiation and chemicals
• Research Institution: National Cancer Center Japan (2014-2016); Hiroshima University (2013)
• Principal Investigator: Shiotani Bunsyo 国立研究開発法人国立がん研究センター, 研究所, 主任研究員 (10627665)
• Project Period (FY): 2013-04-01 – 2017-03-31
• Keywords: ATR / DNA損傷応答 / DNA複製ストレス

Outline of Final Research Achievements

ATR is a kinase that regulates the DNA damage response and maintains genome stability. ATR has been shown to respond to a wider range of DNA damage and exhibit diverse functions. In this study, we aimed to clarify the mechanism underlying DNA structure-dependent activation of ATR and to identify the direct substrate of ATR. As a result, we found a novel ATR activation mechanism at the DNA double strand break site. We also made analog susceptible ATR mutants and successfully detected and identified many unknown substrates.

Free Research Field

DNA損傷応答