The roles of dormant genes in muscle stem cells

2015 Fiscal Year Final Research Report

• Project/Area Number: 25702044
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Partial Multi-year Fund
• Research Field: Applied health science
• Research Institution: Osaka University
• Principal Investigator: Fukada So-ichiro 大阪大学, 薬学研究科(研究院), 准教授 (20432445)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 骨格筋 / 筋衛星細胞

Outline of Final Research Achievements

Muscle stem cells express calcitonin receptor, but its physiological role was unclear. We generated Calcr conditional KO mice and analyzed muscle stem cell and muscle regeneration ability. Intriguingly, the mice exhibited the decreased number of MuSC and impaired regenerative potential. We also generated Hey1-HeyL conditional KO mice, and found the ectopic expression of myogenic genes, which suggests the cell-autonomous role of Hey1-HeyL in muscle stem cells. Using microarray analyses, Nrf2 was identified as the down stream target of Notch and Hey1-HeyL. Nrf2 target genes were downregulated in Nrf2-KO mice, but the KO mice showed no abnormality in muscle stem cells and acute muscle injury model.

Free Research Field

骨格筋生物学