Evaluation a novel molecular mechanism of protecting colon cancer by the foo-derived components

2015 Fiscal Year Final Research Report

• Project/Area Number: 25750050
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Eating habits
• Research Institution: Kyoto Prefectural University of Medicine
• Principal Investigator: Higashimura Yasuki 京都府立医科大学, 医学(系)研究科(研究院), 助教 (70628924)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 短鎖脂肪酸 / プロピオン酸 / 大腸 / PPARalpha / MAPキナーゼ

Outline of Final Research Achievements

Short chain fatty acids (SCFA) are produced in the colonic lumen mainly by bacterial fermentation of dietary fiber. Emerging evidence shows that SCFA has important physiological and pathophysiological effects on colonic and systemic events. Recently, SCFA is regarded as an effective tool for cancer prevention including colon cancer. However, the effect of SCFA on the physiological functions of colonic epithelial cell is unknown. Results show that propionate, known as a kind of SCFA, induced PPARa expression time-dependently and concentration-dependently in YAMC (a mouse intestinal epithelial cell line) cells. The expression levels of PPARa-responsive genes such as carnitine palmitoyl transferase II (CPTII) and trifunctional protein a (TFPa) were up-regulated in the presence of propionate, thereby suppressing triglyceride (TG) accumulation. Furthermore, propionate-mediated PPARa induction required phosphorylation of extracellular signal-regulated kinase.

Free Research Field

生化学