Pseudo-ischemia induced axonal mitochondria changes in cultured dorsal root ganglion neurons

2015 Fiscal Year Final Research Report

• Project/Area Number: 25750207
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Rehabilitation science/Welfare engineering
• Research Institution: Sapporo Medical University
• Principal Investigator: Kikuchi Shin 札幌医科大学, 医学部, 助教 (20404585)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 末梢神経 / ミトコンドリア / 初代培養細胞 / コバルト

Outline of Final Research Achievements

In this study, we investigated that the effects of cobalt, considered to an inducer of hypoxia-inducible factor-1a (HIF-1a), on the axonal degeneration and mitochondrial dynamics in cultured peripheral nervous axons. Twenty-four hours exposure of cobalt decreased the rate of the motile mitochondria in the axons. Of particular note, the 800μM cobalt density suppressed the axonal mitochondria transport completely. The observation of microstructure with the transmission electron microscope found that high density cobalt destroyed the architecture of axonal mitochondria. In the axons treated with cobalt, the number of axonal swelling was associated with the increase of cobalt concentration. Although the mechanisms of neurotoxicity induced by cobalt are not clear, our results showed that high cobalt concentrations reduced mitochondria transport and induced axonal degeneration.

Free Research Field

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