Development of Digital Cell Manipulation Station for Massively Parallel Analysis of Single Cells

2014 Fiscal Year Final Research Report

• Project/Area Number: 25820087
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Intelligent mechanics/Mechanical systems
• Research Institution: Toyohashi University of Technology
• Principal Investigator: NAGAI Moeto 豊橋技術科学大学, 工学(系)研究科(研究院), 助教 (00580557)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Keywords: 細胞操作 / 細胞内デリバリー / マニピュレータアレイ / ナノニードルアレイ / 細胞機能解明

Outline of Final Research Achievements

The aim of the study is to develop a cell manipulation system capable of handling single cells at massively parallel manner (over 10^4 cells) named as “iCellStation” toward understanding the mechanism of diseases and establishing medical treatments. For establishment of fundamental technologies of the cell manipulation system, I achieved the following three research items: (1) Parallel manipulation technique of single cells based on electro-osmotic flow. (2) Single-cell manipulation technique using dielectrophoresis with dielectric coated electrodes. (3) Fabrication technology of nanoneedle array for massively intracellular delivery. Basic technologies for massively parallel cell patterning and intracellular delivery have been developed.

Free Research Field

マイクロ・ナノ工学