Functional analysis of novel miRNA and molecule regulating immune responses.

2015 Fiscal Year Final Research Report

• Project/Area Number: 25830062
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Laboratory animal science
• Research Institution: Nagasaki University (2014-2015); Tokyo University of Science (2013)
• Principal Investigator: YONEZAWA, Tomo 長崎大学, 医歯薬学総合研究科(薬学系), 准教授 (60515964)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: miRNA / 新規分子 / 炎症応答

Outline of Final Research Achievements

We found miRNAs and novel molecules regulating inflammatory responses. To examine whether or not novel molecule, TRIM39R regulates immune responses, we established two gain-of-function models in cultured cells. Then, we performed microarray analysis on the models. The results indicates that TRIM39R significantly regulates genes involved in type I interferon pathway and its anti-viral effects. Furthermore, we confirmed that it actually induces immune responses, such as NFkB and IRF, using luciferase-based reporter assay. We also demonstrated novel miRNA involved in immune responses, mir-125b regulates its target, ADAMTS-4 mRNA according to miRNA dogma by luciferase-based assay and site-directed mutagenesis.

Free Research Field

分子生物学