2014 Fiscal Year Final Research Report
Cell-to-cell communication between tumor cell and their microenvironment via exosomes
| Project/Area Number |
25830089
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Tumor biology
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| Research Institution | Tokyo Medical University |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Keywords | がん微小環境 / エクソソーム / miRNA |
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| Outline of Final Research Achievements |
The aim of this study is to clarify the role of tumor-derived exosome in their microenvironment using multiple myeloma cell lines which show continuous growth in vitro under chronically hypoxic conditions (hypoxia-resistant MM cells; HR-MM cells). We isolated the exosomes from HR-MM cells and assessed exosomal miRNA profiling using the TaqMan low-density array. Endothelial tube formation assay was used for validating function of MM cell-endothelial cell (HUVECs) communication. We found miR-135b was significantly up-regulated in exosomes from HR-MM cells compared to those in the parental cells. The exosomal miR-135b directly suppressed its target, factor inhibiting HIF-1 (FIH-1), in endothelial cells, and enhanced endothelial tube formation under hypoxia. HR-MM cell-derived exosomes could enhance density of CD31 positive endothelial cells in Matrigel plug. Suggesting that the exosomal miR-135b might therefore be one of promising target for controlling angiogenesis in MM.
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| Free Research Field |
腫瘍生物学、発生工学
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