Identification and functional analysis of phosphotyrosine proteins in scirrhous gastric cancer

2015 Fiscal Year Final Research Report

• Project/Area Number: 25830096
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Tumor biology
• Research Institution: National Cancer Center Japan
• Principal Investigator: Shirakihara Takuya 国立研究開発法人国立がん研究センター, 研究所, 研究員 (30548756)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: スキルス胃がん / チロシンリン酸化 / 受容体型チロシンキナーゼ

Outline of Final Research Achievements

We aimed to identify some novel phosphotyrosine proteins related to development of Scirrhours gastric cancer. Although coculture of gastric cancer cells with surrounding cells was performed, none of apparent phosphorylation depend on coculture were detected. So next we tried to reveal downstream effectors of receptor tyrosine kinases such as Met or FGFR2 in diffused type gastric cancers. Proteins purified using anti-receptor antibody and anti-phosphotyrosine antibody, then identified by mass spectrometry. Finally, some data of functional analysis indicates these identified proteins have important roles in malignancy of Scirrhous gastric cancer.

Free Research Field

分子細胞生物学