Paclitaxel resistance-associated proteins in lung cancer cell lines are detected by proteome analyses

2015 Fiscal Year Final Research Report

• Project/Area Number: 25830104
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Tumor diagnostics
• Research Institution: Kyoto Prefectural University of Medicine
• Principal Investigator: Shimomura Masanori 京都府立医科大学, 医学(系)研究科(研究院), 助教 (90433268)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: パクリタキセル耐性 / プロテオーム解析

Outline of Final Research Achievements

We evaluated the function of paclitaxel resistance-associated proteins detected by proteome analyses in human lung cancer cell lines. Affinity purification by paclitaxel immobilized beads revealed several specific bands in paclitaxel resistant cell line; these bands were not revealed in the other cell lines. We can especially identify a protein, FIGNL1, of these specific proteins. As a result of knockdown of FIGNL1 expression using siRNA, the resistant cell line had survival rate of only 60% on the low concentration paclitaxel.

Free Research Field

腫瘍生物学