2016 Fiscal Year Final Research Report
Development of a in vivo introduction method of large genes using the artificial chromosome vector
| Project/Area Number |
25830137
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Medical genome science
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| Research Institution | Tottori University |
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Principal Investigator |
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| Project Period (FY) |
2014-02-01 – 2017-03-31
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| Keywords | 人工染色体ベクター / 濃縮 |
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| Outline of Final Research Achievements |
Human artificial chromosome (HAC) vectors have the ability to delivery a huge gene since there is no size limitation for the DNA to be introduced into them. However, the transfer efficiency of HAC to the target cells is low. The transfer method is based on the microcell-mediated chromosome transfer. Therefore, the concentration of the microcells containing only HAC (HAC-MC) is effective to improve the transfer efficiency. In this study, we successfully established a novel method to concentrate HAC-MC. This method might be also useful for the development of a novel in vivo introduction method of HAC to embryos and individuals.
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| Free Research Field |
細胞工学
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