Identifying cancer cell evolution from early stages of cancer progression by single-cell sequencing

2014 Fiscal Year Final Research Report

• Project/Area Number: 25830141
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Medical genome science
• Research Institution: National Cancer Center Japan
• Principal Investigator: MAMORU Kato 独立行政法人国立がん研究センター, 研究所, ユニット長 (40391916)
• Co-Investigator(Renkei-kenkyūsha): TATSUHIRO Shibata 独立行政法人国立がん研究センター, 研究所, 分野長 (90311414)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Keywords: 一細胞シークエンス / 次世代シークエンサー / がんゲノム / がん細胞進化

Outline of Final Research Achievements

It is important to reveal how cancer is generated and progressed since this leads to better cancer prevention and treatment. Tumor progression can be viewed as a Darwinian evolution, where only fitter cancer cells proliferate more. The simplest way to analyze this process is trace the genetic changes of single cells along time. We used a mouse model for human colon cancer and extracted the genomes of 30 single cells at each of the 3 time points along tumor progression. An algorithm to identify single cell's variants is necessary for analyzing single cell genomes and we developed such an algorithm, using single cell sequencing data.

Free Research Field

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