2015 Fiscal Year Final Research Report
Bacterial genomic DNA protection mechanism against oxidative stresses
| Project/Area Number |
25840002
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Molecular biology
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| Research Institution | University of Tsukuba |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Keywords | 核様体 / 酸化ストレス / 細菌 / 染色体 |
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| Outline of Final Research Achievements |
The protection of genomic DNA against oxidative stresses is essential to sustain the living cells including symbiotic/pathogenic bacteria inhabiting human. Here, I studied the molecular mechanisms to protect bacterial genomic DNA. I focused on about ten proteins which I have shown to be equipped on the the bacterial nucleoid which is the complex of genomic DNA, protein, etc. Among the proteins examined by techniques of bacterial molecular genetics, Dps protein in an opportunistic pathogen Staphylococcus aureus exhibited that its DNA binding and nucleoid clogging abilities are not sufficient to protect genomic DNA and cells, and that its ferroxidase activity (ferric iron chelating ability) is essential.
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| Free Research Field |
分子生物学・細菌遺伝学
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