2015 Fiscal Year Final Research Report
Structural basis for small RNA functional mechanism in eukaryotes
| Project/Area Number |
25840019
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Structural biochemistry
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| Research Institution | Niigata University |
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Principal Investigator |
Miyoshi Tomohiro 新潟大学, 研究推進機構超域学術院, 助教 (60534550)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
|
| Keywords | Argonaute / small RNA |
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| Outline of Final Research Achievements |
Argonaute proteins are widespread in all domains of life from bacteria to eukaryotes, and are an essential component of the nucleic acid-based silencing systems. Although many aspects of the structure of Argonaute-nucleic acid complexes have been described in previous crystal structure studies, the recognition mechanism of the guide and target strands by Argonaute proteins is still not fully understood. In the present study, we have solved the structure of Argonaute in complex with guide RNA and target DNA by X-ray crystallography.
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| Free Research Field |
構造生物学、分子生物学
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