2014 Fiscal Year Final Research Report
Structure changes of the novel ABC-type protein complex involved in the iron-sulfur cluster biosynthesis
| Project/Area Number |
25840023
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Structural biochemistry
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| Research Institution | University of Miyazaki |
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Principal Investigator |
WADA Kei 宮崎大学, テニュアトラック推進機構, 助教 (80379304)
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Keywords | 鉄硫黄クラスター / 結晶構造解析 / 立体構造 / 鉄硫黄蛋白質 / ABC蛋白質 |
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| Outline of Final Research Achievements |
In this study, the quaternary structure of theSufB2-SufC1-SufD2 complex involved in Fe-S cluster biogenesis in which two SufC molecule are bound to the C-terminal domain of SufB/SufD was determined. The central domain of SufB and SufD possess the novel structural motif. Biochemical and physiological analyses based on the crystal structure provide unprecedented insights into the correlation of cluster assembly with the dynamic conformational change driven by ABC-ATPase activity. We propose a molecular mechanism of Fe-S cluster biogenesis for SufBCD complex.
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| Free Research Field |
構造生物学
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